Publications on Shape Analysis in Medical Imaging :

• R. Eils et al. :
  • Aspects of 3D chromosome reorganization during the onset of human male meiotic prophase, 1998.
  • 3D reconstruction of painted human interphase chromosomes: Active & inactive X-chromosome territories have similar volumes but differ in shape & surface structure, 1996.
  • Application of Confocal Laser Microscopy & 3D Voronoi Diagrams for Volume & Surface Estimates of Interphase Chromosomes, 1995.
• Y. Kergosien et al. :
  • Topology & Visualization: From Generic Singularities to Combinatorial Shape Modeling, 1992.
  • Generic sign systems in medical imaging, 1991.

The 3D morphology and distribution of human chromosomes 3 were studied in nuclei of spermatogonia and spermatocytes I from formaldehyde-fixed human testis sections. Chromosome arms, pericentromeres and telomeric regions were painted by a three-color, five-probe fluorescence in situ hybridization protocol. Light optical serial sections of premeiotic and meiotic nuclei obtained by confocal laser scanning microscopy revealed that premeiotic chromosomes 3 are separate from each other and occupy variably shaped territories, which are sectored in distinct 3 p- and q-arm domains. 3D reconstructions of the painted chromosome domains by a Voronoi tessellation approach showed that mean chromosome volumes did not differ significantly among the premeiotic and meiotic stages investigated. A significant increase in surface area and reduction of dimensionless 'roundness factor' estimates of arm domains indicated that the restructuring of spatially separate chromosome territories initiates during preleptotene. Telomeric regions, which in meiotic stem cells located predominantly in arm-domain chromatin, showed a redistribution towards the domain surface during this stage. At leptotene homologues were generally misaligned and displayed intimate intermingling of non-homologous chromatin. Pairing initiated at the ends of bent zygotene chromosomes, which displayed a complex surface structure with discernible sister chromatids. The results indicate that, in mammals, homology search is executed during leptotene, after remodeling of chromosome territories.

Keywords: Meiosis, Fluorescence in situ hybridization, FISH, Human, Spermatogenesis, Homology search, Chromosome pairing.

Keywords : Color Computer Graphics; Computerized Tomography; Data Reduction; Differentiable Topology; Image Data Sets; Imaging Techniques; Medical Applications; Medical Imaging Semiotics; Morse Theory; Projection Imaging; Sectional Imaging.

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